Title: Effects and Mechanisms of Pioglitazone on Expression of Glucose Transporter-4 in Cardiac Muscle Tissue of Rats with Diabetes Mellitus
Abstract:OBJECTIVE: To observe the effects of long-term administration of pioglitazone at different doses on the expression of glucose transporter-4(GLUT-4) of cardiac muscle tissue in rats and to discuss the ...OBJECTIVE: To observe the effects of long-term administration of pioglitazone at different doses on the expression of glucose transporter-4(GLUT-4) of cardiac muscle tissue in rats and to discuss the possible mechanisms.METHODS: A total of 40 Wistar rats were enrolled,of which,10 were randomly assigned to normal control group(NC),and the other 30 were injected intraperitoneally with streptozotocin to establish diabetes mellitus(DM) rat model.After successful modeling,the rats were assigned to DM model group(DM,normal saline),high and low dose pioglitazone group(HP,LP;0.75,0.25 mg·kg-1·d-1).The NC groups received normal saline for 12 weeks.Blood glucose(BG) level before grouping(baseline level) and prior to death was determined by tail-cutting method.GLUT-4 level in cardiac muscle tissue and both protein and mRNA expression of AMPKα were determined by RT-PCR and western blot,respectively.RESULTS: As compared with NC group,the BG level in DM group was significantly increased but the expression of GLUT-4 and AMPKα decreased significantly(P0.01).As compared with DM group,the differences of BG level in HP and LP groups were not significant,and the GLUT-4 and AMPKα expression were markedly increased(P0.01).CONCLUSIONS: Pioglitazone treatment resulted in an increased expression of GLUT-4 in cardiac muscle tissue of DM rats,the underlying mechanism is possibly attributed to its effect in up-regulating the expression of AMPKα.Read More
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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