Title: Development of Monoclonal Antibodies Against VP1 Protein of Chicken anemia virus and Its Identification of Epitopes
Abstract: Female Balb/c mice of 6~8 weeks were immunized by the purified recombinant Chicken amemia virus(CAV)fusion protein.After 3 immunizations,the spleen cells from immunized mice were fused with mouse myeloma cells SP2/0.Six positive hybridoma cell lines stably secreting monoclonal antibodies(MAbs)were selected by 3 cycles of limited dilutions.All MAbs reacted with MDCC-MSB1 cell infected with CAV by indirect immunofluorescence assay(IFA)and they recognized the VP1 protein expressed in Baculovirus by Western blot.It demonstrated that all MAbs possesed good specificity.The 6 MAbs were IgG1 subtype and light chains of all MAbs were kappa.Taking advantage of VP1 fragments expressed,the epitopes corresponding to the MAbs were analyzed.The results showed that the monoclonal antibodies 1C5,2F3,4D2 and 4E8 recognized the same epitope,which was located in 218~274 aa.Moreover,the two other epitopes were identified and they were located in 274~301 aa and 324~369 aa,respectively.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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