Title: Production and characterization of monoclonal antibody to recombinant chicken IL-2
Abstract: Four hybridoma cell lines named as 1A12,4A3,3E7 and 4E1,secreting monoclonal antibody (MAb) against chicken IL-2 were produced by fusing mouse myeloma cells (SP2/0) with spleen cells from BALB/c immunized with the purified recombinant chicken IL-2 protein. The four MAbs were identified as follows:The ELISA titers were 5.12×10~5,1.02×10~6,2.56×10~5 and 4.09×10~6,respectively.All the Mabs falled into type IgG1. Western-blot analysis showed that the four MAbs could react with chicken IL-2.
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
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Cited By Count: 1
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