Title: Preparation of monoclonal antibodies against N protein of equine arteritis virus
Abstract: To prepare monoclonal antibodies(MAbs) against equine infectious anemia virus(EAV) N protein,the SP2/0 cells were fused with spleen cells of BALB/C mice immunized with the purified recombinant N protein,two hybridoma cell lines stably secreting antibody was obtained by indirect ELISA.The two MAbs was identified to be IgG1/κ.Western blot analysis showed that the two MAbs reacted positively with EAV.The MAbs developed could serve as a tool for further study on serum research.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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