Title: Determination of Berberine Hydrochloride in Wuji Pills by HPLC
Abstract: Objective:To establish a method for the determination of berberine hydrochloride in Wuji Pills.Methods: The determination was performed by high performance liquid chromatography(HPLC).The chromatographic column was Gemini C18(4.6 mm×250 mm,5 μm).The mobile phase was Acetonitrile-0.05 mol/L KH2PO4(35∶65,pH5.0).The wave lenghth was 246 nm.The flow rate was 1.0 ml/min.The column temperature was 25℃.Results:Berberine hydrochloride was separated completely.The mean recovery rate was 97.82% and RSD was 0.80%.Conclusion:This method is simple,rapid and accurate.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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Cited By Count: 2
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