Title: Screening of SARS-CoV N Protein Mimotopes from Phage Display Peptide Library
Abstract: Objective To obtain the bioactive mimicry peptide of SARS N ptotein. Methods Monoclonal antibodies of SARS-CoV N protein was used to screen the phage display random 12-peptides library. The positive clones were identified by sandwhih ELISA and competitive ELISA. Results Positive clones were obtained from the screening. Two different mimicry epitopes were confirmed by DNA sequencing as mimotope of SARS N protein: GXLXTPXXXXGT, and TTLPXXXXAXXX. Conclution SARS N protein mimotopes were obtained from the screening of a 12-mer phage peptide library. The results provide a new approach for SARS viral therapy and vaccine development.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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