Title: Submerged Culture of Mycelium of Ectomycorrhizal Basidiomycete Tricholoma matsutake
Abstract:Objective To investigate the method and optimum conditions of Tricholoma matsutake mycelium fermentation.Methods Dynamic changes of biomass,residual glucose concentration and pH in submerged culture w...Objective To investigate the method and optimum conditions of Tricholoma matsutake mycelium fermentation.Methods Dynamic changes of biomass,residual glucose concentration and pH in submerged culture was investigated with Tricholoma matsutake stain,which was isolated and identified by RAPD-PCR method.By comparing the effect of different inoculum ages on biomass,the optimum inoculum age was determined.Based on comparing the effect of different initial glucose concentrations on dynamic changes of fermentation,the optimum time of feeding glucose was established by carrying out the feeding culture strategy in submerged culture.Results The optimum inoculum age was 6 days.The lower initial glucose concentration obviously availed to shorten the lag phase of the growing of mycelium.And the higher initial glucose concentration made the mycelium maintain growth for a long period of time and finally reached a higher mycelium yield.The d 12 was confirmed as the optimum feeding time.By applying the feeding culture strategy,12.77g/L mycelium biomass was obtained on day 24 in flask system.Conclusion The method and optimum conditions is feasible for accumulating the mycelium of Tricholoma matsutake in submerged culture.Read More
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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