Title: Simultaneous Determination of Eight Components in Compound Huangkui Granules by HPLC
Abstract:Objective: To establish an HPLC method for simultaneously determining the contents of calycosin-7-O-β-D-glucoside, hypericin, rutin, isoquercetin, myricetin, quercetin, loganin, morroniide in compound...Objective: To establish an HPLC method for simultaneously determining the contents of calycosin-7-O-β-D-glucoside, hypericin, rutin, isoquercetin, myricetin, quercetin, loganin, morroniide in compound Huangkui granules. Method: The determination was performed on an Aglient C18( 4. 6 mm × 250 mm,5 μm) chromatographic column, the mobile phase was acetonitrile-0. 05% formic acid aqueous solution with gradient elution at a flow rate of 1. 0 m L·min-1; the detection wavelength was 240,260 and 360 nm,column temperature was 30 ℃. Result: The calibration curves of calycosin-7-O-β-D-glucoside, hypericin, rutin,isoquercetin,myricetin,quercetin,loganin,morroniide were linear in the ranges of 3. 5-35. 0( r = 0. 999 6),32. 09-300. 9( r = 0. 999 7),2. 21-22. 12( r = 0. 999 7),18. 68-186. 9( r = 0. 999 8),6. 32-63. 20( r =0. 999 7),3. 54-35. 44( r = 0. 999 6),12. 39-123. 9( r = 0. 999 6),12. 88-128. 8 mg·L-1( r = 0. 999 6),respectively. The average recoveries were 98. 1%( RSD 1. 7%),99. 7%( RSD 0. 6%),98. 7%( RSD2. 5%),100. 1%( RSD 1. 4%),99. 7%( RSD 1. 0%),99. 9%( RSD 2. 7%),99. 0%( RSD 1. 2%),99. 7%( RSD 1. 3%),respectively. Conclusion: The method established is simple,accurate and reproducible,which can be used for the quality control of Huangkui granules.Read More
Publication Year: 2015
Publication Date: 2015-01-01
Language: en
Type: article
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