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Title: $Simultaneous determination of contents of five constituents in Anshenbuxin tablets by RP- HPLC
Abstract: Objective: To establish a RP- HPLC method for determination of sodium danshensu,salidroside,2,3,5, 4'- tetrahyroxystilbene- 2- O- β- D glucoside( hereinafter referred to as stilbene glucoside),nuzhenide and salvianolic acid B in Anshenbuxin tablets( traditional Chinese medicines). Methods: The HPLC separation was achieved on a Diamonsil C 18 column( 4. 6 mm × 200 mm,5 μm) at 30 ℃. The mobile phase consisted of acetonitrile- 0. 1% phosphoric acid solution with gradient elution at a flow rate of 1. 0 mL·min- 1. The UV detection wavelength was set at 220 nm. Results: The linear ranges of sodium danshensu,salidroside,stilbene glucoside,nuzhenide and salvianolic acid B were 11. 94- 119. 4 mg·L- 1( r = 0. 9994,n = 6),5. 520- 55. 20 mg·L- 1( r = 0. 9997,n = 6), 2. 848- 28. 48 mg·L- 1( r =0.9992,n =6),1. 104-11. 04 mg·L- 1( r =0. 9990,n =6) and 51. 78-517. 8 mg· L- 1( r =0. 9993,n =6),respectively. The average recoveries( n =6) of sodium danshensu,salidroside,stilbene glucoside,nuzhenide and salvianolic acid B were 100. 1%( RSD = 1. 8%),99. 7%( RSD = 1. 8%),101. 1%( RSD = 1. 2%),100. 8%( RSD = 1. 8%) and 100. 1%( RSD = 0. 9%),respectively. Conclusion: The method is simple, accurate,repeatable and can be used for quality control of Anshenbuxin tablets.