Title: Separation and Purification of the Flavonoids from Sarcandrae glabra with Macroporous Resin
Abstract: Objective A method for separation and purification of the flavonoids from Sarcandrae glabra with macroporous resin was studied.Methods By using Sarcandrae glabra in Guizhou and with the content and recovery rate of flavonoids as indexes and the static absorption capacity and the static elution ratio were observed. Results The results were as flollows: The static absorption capacity of AB-8 type resin was 116.25 mg·g-1, the static elution ratio was 92.9%, the dynamic absorption capacity of AB-8 type resin was 94.5 mg·g-1, the recovery rate was more than 96.1% and the purity was more than 90%. AB-8 type resin was the best for separating and purificating Sarcandrae glabra in flavonoids.Conclusion The optimum conditions is: AB-8 type macroporous resin, the eluant is 70% alcohol and 3 times as the volume of the resin, the volume of the resin is 10.5 times of flavonoids in sample, concentration of flavonoids of sample is 19.8 mg·ml-1 and current velocity of 2~3 ml·min-1, pH value of sample is 4~5.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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