Title: Optimization of Purification Technology for Total Flavonoids from Salvia plebeia by Macroporous Adsorption Resin
Abstract: Objective:To optimize enrichment and purification technology of total flavonoids from Salvia plebeia.Method:With adsorption-desorption ratio and transfer ratio as indexes,optimum macroporous resin was selected by static adsorption-desorption ratio tests;The concentration of eluting agent was determined with the content of total flavonoids as index;Purification technology was optimized with impurity rate,transfer rate of homoplantaginin and transfer rate of total flavonoids as indexes.Result:D101 was selected for its excellent adsorption and desorption properties,optimum process was as follows:the amount of crude drugs to D101 macroporous resin 1 g · mL-1,eluted with 7 BV 75% ethanol,elution flow rate 6 mL · min-1.Conclusion:Total flavonoids from S.plebeia could be effectively purificated and separated by D101 macroporous resin.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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Cited By Count: 2
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