Title: Growth Inhibition of a Selective PPAR-γ Inhibitor,T0070907,in Nasopharyngeal Carcinoma Cells
Abstract: 【Objective】PPAR-γ(peroxisome proliferator-activated receptor-γ)antagonists have been documented to induce cancer cell proliferation inhibition and apoptosis.In the present study PPAR-γ's effect on cell growth of NPC cell lines and the possible mechanism was investigated via its selective inhibitor T0070907.【Methods】PPAR-γmRNA and protein expression in five human NPC cell lines(CNE1,CNE2,HONE1,SUNE1,and 5-8F)was detected with RT-PCR and Western blotting analysis.NPC cells growth inhibition by T0070907 was measured by MTT assay,and cell cycle arrest of NPC cells treated with T0070907 was assayed by fluorescence-activated cell sorter(FACS)analysis.Cell cycle-associated protein expression was detected by Western blotting.【Results】PPAR-γwas expressed both at mRNA and protein levels in five NPC cell lines.NPC cells'proliferation was significantly inhibited by T0070907,and the growth inhibition was dose-and time-dependent.CNE1 and CNE2 cells treated for 48 h with T0070907 markedly accumulated dose-dependently in the phase of G2/M,while they differed significantly(P0.05)in the phase as well.T0070907 suppressed expression of cell cycle-related proteins including Cyclin A,Cyclin B1,Cdc2,and Cdk2 but increased pCdc2 expression.【Conclusion】PPAR-γinhibitor T0070907 could induce proliferation inhibition of NPC cells to the phase of G2/M,and this cell cycle arrest might result probably from regulation of Cyclin A,Cyclin B1,Cdc2,Cdk2,and pCdc2.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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