Title: Tanshinone IIA induces apoptosis in human nasopharyngeal carcinoma CNE1 cells
Abstract: Objective To explore the effect of anshinoneⅡA(Tan ⅡA) on induction of apoptosis in human nasopharyngeal carcinoma CNE1 cells and on expression of Bcl-2 gene and COX-2 promoter.Methods CNE1 cells were cultured in vitro and treated with various concentrations of TanⅡA.The cytotoxicity effect of Tan ⅡA against CNE1 cells was evaluated by MTT assay.The cell cycle distribution,apoptotic rate and Bcl-2 expression were investigated by flow cytometry.COX-2 promoter activity was detected by the dual luciferase assay.Results TanⅡA treatment inhibited CNE1 cell proliferation in a concentration-and time-dependent manner(P0.01).Compared with the control group,TanⅡA groups had significantly higher apoptosis rates(P0.01),and the apoptosis rates increased with the increase of Tan ⅡA concentration;the cells were blocked in the G2/M phase.TanⅡA inhibited Bcl-2 protein expression in a concentration-dependent manner within 6 h after treatment(P0.05).Bcl-2 expression was initially increased and then decreased with the increase of TanⅡA concentrations(P0.05) 24 h after treatment.Activity of COX-2 promoter decreased with the increase of TanⅡA concentration(P0.01).Conclusion TanⅡA can inhibit the growth and promote the apoptosis of CNE1 cells;inhibition of Bcl-2 expression and COX-2 activity might be one of the mechanisms for the TanⅡA-induced CNE1 cell apoptosis.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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Cited By Count: 1
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