Title: CONSTRUCTION OF OMP25 GENE IN PROKARYOTIC EXPRESSION VECTOR FROM B.MELITENSIS
Abstract: Objective: To explore the function of outer membrane protein OMP25 in Brucella melitensis M5 strain,a prokaryotic expression vector containing omp25 was constructed.Methods: The genome DNA of B.melitensis was isolated with commercial available kit.The PCR product containing omp25 gene was inserted into vector pMD19-T.The recombinant plasmids pMD19-T-omp25 was transformed into competent E.coli DH5α.Then the omp25 gene fragment was cut out and cloned into the prokaryotic vector pET-32a which was transformed into E.coli DH5α.Results: An expected gene fragment was isolated from the bacterial genome by PCR amplification and confirmed with sequencing.Conclusions: The recombinant expression plasmid pET-32a-omp25 was correctly constructed,which could provide the materials for OMP25 function analysis.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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