Title: Construction of the prokaryotic expression vector for EBV BMRF1 gene
Abstract: Objective To Construct a prokaryotic expression vector for EBV BMRF1 gene,which should provide the condition for expressing the protein.Methods The BMRF1 gene was amplified from the EBV strain B95-8 cell line with specific primers.After digested with BamHⅠand XhoⅠ,The PCR product was inserted into the prokaryotic expression plasmid pGEX-5T.The recombinant plasmid was confirmed by restriction enzyme analysis and DNA sequencing.Results The length of specific fragment amplified by PCR was 1 233 bp.Double digestion of the constructed plasmid revealed DNA fragments of expected size.The sequence of the inserted BMRF1 gene was conformed by blasting to GenBank.Conclusion The recombinant prokaryotic expression vector pGEX-5T-BMRF1 was successfully constructed.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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