Title: Cloning and construction of prokaryotic expression vector for the beta-hemolysin of Staphylococcus aureus
Abstract: One pair of specific primers to hlb gene of S.aureus was designed and synthesized according to the published sequence in GenBank,a DNA encoding beta-hemolysin(hlb)was amplified by PCR and inserted it into pMD18-T.The recombinant plasmid of pMD-T-β was transformed into host BL-21 bacterium.The recombinant plasmid of pET-32a-βcontaining hlb gene were identified by restriction enzymtic analysis,PCR examination and sequence analysis.The result indicates that the recombinant PET-32a-β prokaryotic expression vector has been successfully construted.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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