Title: Determination the concentration of cyclosporine A in whole Blood by SPE-HPLC
Abstract: Objective To establish an HPLC method for assay of cyclosporine A in whole blood.Methods The chromatographic column was Nucleodur C8(150 mm×4.6 mm,5 μm),the mobile phase consisted of acetonitrile and water(71∶29,v/v),the column temperature was controlled at 65 ℃ and the UV detector was operated at 210 nm.The whole boold samples were added the 80% acetonitrile precipitate albumen,after the SPE subminiature column was disposed,the sample was directly injected into the system.Results The calibration curve was linear in the range of 40.0~1 600.0 ng·mL-1.The average recoveries of the three concentrations were all higher than 95.0%.The intra-day RSD and inter-day RSD was both less than 10.0%.the extraction recovery of cyclosporine A was higher than 86.0%.Conclusion The method is steady and accurate.It is suitable for measuring cyclosporine A in whole blood.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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