Title: Determination of Cyclosporine Whole Blood by High Performance Liquid Chromatography
Abstract: A HPLC method for the assay of cyclosporine A in whole blood was estabtished.After multistep pretreatment ,20 μL sample was injected into reversed phase column (200 mm ×4.6 mm,5 μm,C 18 ) at 75℃,eluted with a mobile phase of acetonitrile/water(80∶20) at a flow rate of 1.0 mL/min and monitored at 208 nm.The analytical recovery was 99 %~104% and inter day and intra days RSD was below 3.4% and 8.2% respectively.The detection limit was 25 μg/L.The method provided excellent recovery and good precision,and was accurate.No interference was observed.
Publication Year: 2000
Publication Date: 2000-01-01
Language: en
Type: article
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