Title: Correlation of IFN - γ and IL -6 levels produced by peripheral blood mononuclear cells and HBVDNA load in patients with chronic hepatitis B
Abstract: Objective To investigate the different HBVDNA load in chronic hepatitis B(CHB) patients treated with lami-vudine before and after peripheral blood mononuclear cells(PMBC) secretion of IFN-γ,IL-6 level changes and its clin-ical significance.Methods Fluorescence quantitative polymerase chain reaction(FQ-PCR) was used to detect the quan-tity of HBV load and enzyme-liked immunosorbent assay(ELISA) was used to determine the levels of INFγ and IL-6 se-creted by phytohemagglutinin(PHA) stimulated PBMC.Results IFN-γ level was lower but IL-6 level was higher than that of the control group(P 0.01) before lamivudine treatment.After lamivudine treatment,IFN-γ levels were signifi-cantly increased(P 0.01 and P 0.05 respectively),but IL-6 levels were significantly decreased(P 0.01 and P 0.05 respectively) in patients from HBVDNA high and middle level groups.However,in patients from HBVDNA middle and low level group,IFN-γ level was lower but IL-6 level was higher than that of control group(P 0.01).There was a negative correlation between IFN-γ level and the HBVDNA level(r = 0.89,P 0.00 1),but IL-6 level positively corre-lated with HBVDNA level(r = 0.92,P 0.001) and ALT level(r = 0.74,P 0.001).Conclusion IFN-γ and IL-6 levels secreted by PBMC in CHB patients can be used for the judgment of therapeutic effects during lamivudine treatment.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot