Title: Study on effect of antiviral therapy on changes in levels of IL-10 and IL-12 secreted by PBMC in patients with genotypeIb chronic hepatitis C
Abstract: Objective To study the changes in levels of IL-10 and IL-12 secreted by PBMC in patients with genotypeⅠb chronic hepatitis C accepted pegylated interferon and ribavirin therapy,to analyze the correlation between changes in levels of IL-10 and IL-12 and response of treatment,and to study the feasibility of levels of IL-10 and IL-12 as indicators for evaluation of efficacy of antiviral therapy.Methods Sixty cases of chronic hepatitis C with genotypeⅠb were divided into 3 groups,19 cases in low virus load group with virus load of 1.0×103 IU/mlHCV-RNA≤1.0×104 IU/ml,23 cases in medium virus load group with virus load of 1.0×104IU/mlHCV-RNA≤1.0×106 IU/ml and 18 cases in high virus load group with virus load of HCV-RNA1.0×106 IU/ml;and these patients were treated by combination of Pegasys and ribavirin for 48 weeks.Fifteen cases of chronic hepatitis C with negative serum HCV-RNA and 15 healthy blood donors were allocated as control groups.Peripheral blood mononuclear cells were isolated and cultured during pretherapy and different periods in therapeutic course and post-treatment,their secretions were collected after cultivation for 72 hours,and the supernatants were collected after centrifugation.Levels of PBMC IL-10 and IL-12 were determined by ELISA in all cases.Results The level of PBMC IL-10 in chronic hepatitis C patients with positive HCV-RNA was significantly higher than that of control groups(P0.05).The difference in level of PBMC IL-12 compared among three groups was not statistically significant(P0.05).The relationship between change in levels of PBMC IL-10,IL-12 and antiviral responses was positively correlated,the level of PBMC IL-10 was decreased and IL-12 was rapidly increased while the HCV-RNA load was significantly declined.Conclusion Cell immunity of patients with chronic hepatitis C tends to Th2 type;the level of preinflammatory cytokines IL-12 will be quickly increased if antiviral therapy is effective,and the direction of cell immunity will tend to Th1 type;the change in levels of PBMC IL-10 and IL-12 has certain significance in predicting efficacy of antiviral treatment with Pegasys and ribavirin.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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