Title: Content Determination of Quercetin in Different Parts of Polygoni Polygonum perfoliatum
Abstract: OBJECTIVE:To determinate the contents of quercetin in different parts of Polygonum perfoliatum.METHODS:HPLC was performed on DiamonsilTMC-18 column(250 mm×4.6 mm,5μm)with methanol-0.4%phosphatic acid(50:50)as mobile phase at the flow rate of 1.0 mL·min-1.The detection wavelength was set at 360 nm.RESULTS:The linear range of quercetin was 0.05~1.00 μg(r=0.999 8)with an average recovery of 97.73%(RSD=1.03%,n=6).The contents of quercetin were different significantly among different parts of P.perfoliatum.CONCLUSION:The method is simple,accurate and highly reproducible,and can be used for the quality control of P.perfoliatum.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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