Title: Determination of Quercetin in Polygonum capitatum by HPLC
Abstract: Aim To develop a method of RP-HPLC for determination of quercetin content in Polygonum capitatum.Methods RP-HPLC analysis was carried out by using Hypersil BDS C18 column(4.6mm×200mm,5μm)and methanol-water-phosphoric acid (50∶50∶0.4,V/V/V) as the mobile,with UV detection wavelength at 370nm.Results The method was linear over the range of 0.04~0.16μg of quercetin (r=0.999 8).The average recovery was 101.17%,with RSD of 1.42% (n=9).Conclusion The method is simple,accurate,highly sensitive and reproducible.It may be used for the quality determination of quercetin in Polygonum capitatum.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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Cited By Count: 1
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