Title: Determination of betulin in Fructus ligustri lucidi by RP-HPLC
Abstract: The method with different solvents for exacting betulin from Fructus ligustri lucidi was investigated via HPLC on Supelcosil LC-PAH C18(250 mm×4.6 mm,5 μm),mobile phase [830 mL acetonitrile∶170 mL water∶1 mL H3PO4],the flow rate 1.0 mL/min,the detection wavelength at 210 nm.The content of Betulin was determined with external standard method.The yield of betulinic was higher with ultrasonics in 1-Butanol than that with others.The standard curves of Betulin showed good linearity over the range of 2-20 μg and the average recovery was 98.87% with RSD 1.91%(n=5).The method is simple,stable,accurate and reliable for the quality control of Fructus ligutri lucidi.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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