Title: Determination of nvzhenoside in Fructus Ligustri lucidi by RP-HPLC
Abstract: Objective To establish an HPLC method for the determination of nvzhenoside in Fructus Ligustri lucidi.Methods The separation was performed on an Kromasil C18 column (250 mm×4.6 mm,5 μm) with the mobile phase consisting of acetonitrile-water(20:80,v/v) and the detective wavelength of 240 nm.Results The calibration curve was linear over 25.5-510 μg·mL-1 for linarin(r=0.999 9,n=6).The average recovery was 99.7%(RSD=1.7%,n=9).Conclusion The method is simple,accurate and reproducible,which is suitable for the quality control of nvzhenoside in Fructus Ligustri lucidi.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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