Title: Cloning and identification of the gene encoding the superantigen staphylococcus enterotoxin A
Abstract: Objective To clone the full-length gene of staphylococcal enterotoxin A(SEA) and construct its expression vector.Methods The technique of polymerase chain reaction(PCR) was employed to obtain 771bp full-length sequence of SEA from the gene group DNA of standard strain ATCC13565.The SEA gene was amplified and inserted into cloning vector pUC57.The structure of recombinant pUC57-SEA plasmid was confirmed by restriction endonuclease and sequence analysis.Results The 771 bp DNA sequencing showed that the DNA sequence of the cloned gene was identical to the data recorded in Genbank Database.Conclusion This study has successfully amplified and cloned the full-length gene encoding SEA toxin and established a basis for the SEA gene target for antitumor research.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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