Title: Effect of drug-containing serum of Fushen Jiangzhuo formula on anti-fibrosis factors HGF and BMP-7 in rat renal interstitial fibroblasts with renal tubulointerstitial lesion
Abstract: AIM: To study the protective effects and mechanism of Fushen Jiangzhuo formula( FSJZ) on the rat renal interstitial fibroblasts with renal tubulointerstitial lesion. METHODS: The serum containing FSJZ and blank control serum were prepared. The rat model of mesangial proliferative glomerulonephritis( Ms PGN) was established and extended the time of modeling to 20 th weeks for developing tubulointerstitial lesion naturally. The rat renal interstitial fibroblasts at the end of 12,16,20 week of modeling were isolated and cultured. The effects of FSJZ on the expression of hepatocyte growth factor( HGF) and bone morphogenetic protein-7( BMP-7) at mRNA and protein levels in the pathological renal interstitial fibroblasts were determined. RESULTS: The anti-fibrosis factors HGF and BMP-7 were changed in pathological renal interstitial fibroblasts. Renal tubulointerstitial lesion significantly down-regulated the expression of HGF and BMP-7,while the drug containing serum of FSJZ significantly up-regulated the expression of HGF and BMP-7. CONCLUSION: Drug containing serum of FSJZ has protective effect on pathological renal interstitial fibroblasts by regulating the mRNA and protein expression of HGF and BMP-7.
Publication Year: 2015
Publication Date: 2015-01-01
Language: en
Type: article
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