Title: Role of hepatocyte growth factor in renal interstitial fibrosis in unilateral ureteral obstruction rats
Abstract: Objective:To study the role of hepatocyte growth factor(HGF)in the process of renal interstitial fibrosis caused by unilateral ureteral obstruction(UUO)in rats, and to study the correlation between HGF and transforming growth factor-β 1(TGF-β 1). Methods:Eighteen Spraque-Darley rats were subjected to UUO, and additional eighteen rats were sham operated.Six rats of each group were killed respectively at 3, 7, 14 days after surgery.Immunohistochemistry study was performed on renal tissue for HGF, TGF-β 1, α-smooth muscle actin(α-SMA), and fibronectin. Histological changes were also observed by HE ang Masson staining. Results:In the early stage of renal interstitial fibrosis, expression of renal HGF increased significantly, whereas expression of TGF-β 1 decreased significantly. In advanced stage, expression of renal HGF decreased , whereas expression of TGF-β 1 increased. The expression of HGF was negatively correlated with the expression of TGF-β 1,α-SMA and FN . Conclusion:HGF may play a crucial role in the process of renal interstitial fibrosis. TGF-β 1 level increasing and local HGF expression decreasing may be responsible for the progression of renal interstitial fibrosis.
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
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