Title: Effect of curcumin and TNF-α on vascular endothelial growth factor expression in human lymphoma cells and the angiogenesis
Abstract: OBJECTIVE To investigate the effect of curcumin and tumour necrosis factor(TNF)-α on the expression of vascular endothelial growth factor in Raji cells and their effect on angiogenesis in human umbilical vein endothelial cells(HUVECs)-derived cell line(ECV304).METHODS VEGF secreted by Raji cell line was determined by ELISA.Angiogenesis was tested by network formation of endothelial cells on Matrigel.Levels of VEGF mRNA in Raji cells and Notch1 mRNA in ECV304 cells were measured by RT-PCR.RESULTS The content of VEGF secreted by Raji cells was increased by TNF-α and suppressed by curcumin(P0.01).The VEGF mRNA expression,mainly VEGF165 and VEGF121 in Raji cells(containing 165 and 121 amino acid residues,respectively) was detected in all fractions.TNF-α augmented the expression of VEGF165 and VEGF121 mRNA and curcumin reduced the expression(P0.01).No network and cord were formed in control and curcumin group.There was tube formation on matrigel in the supernatants of Raji culture group and the supernatants groups treated by VEGF and TNF-α in Raji cell.Notch1 mRNA was detected in ECV304 cell line but no difference in VEGF group compared with control(P0.05).CONCLUSION The expression of VEGF in Raji cells was increased by TNF-α and suppressed by curcumin.VEGF and TNF-α can induce angiogenesis and curcumin can inhibit angiogenesis in ECV304 cells.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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