Title: Study on rpoB gene mutations in Mycobacterium tuberculosis
Abstract:Objective To understand rpoB gene mutaions in rifampin (RIF)-resistant Mycobaterium trberculosis(M.TB) strains isolated from tuberculosis (TB) patients in rural area and to provide genetic evidence fo...Objective To understand rpoB gene mutaions in rifampin (RIF)-resistant Mycobaterium trberculosis(M.TB) strains isolated from tuberculosis (TB) patients in rural area and to provide genetic evidence for TB control in rural areas.Methods 145 isolates from two counties were examined for rpoB gene mutations by the polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) method compared with virulent M.TB H_ 37R_v.Results The prevalence of rpoB gene mutation was 71.4% in RIF-resistant or multidrug-resistant M.TB from patients in this rural area.rpoB gene mutations were not found in all the 117 susceptible strains.In comparison with drug susceptibility test results the sensitivity and specificity of PCR-SSCp assay were 71.4% and 100%,respectively.Conclusion RIF-resistance of M.TB is strongly related to rpoB gene mutations.PCR-SSCP assay can detect rpoB gene mutations of RIF-resistant M.TB.It may thus be helpful to include a rapid detection of drug-resistance of M.TB in resistance containment.Read More
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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