Title: Effects of p53 gene on K562 cell line proliferation
Abstract: Objective To investigate effects of p53 gene on proliferation and apoptosis in K562 cell line. Methods P53 gene was transferred to monoclonal K562 cells with electroporation. P53 gene transcription and expression were detected with RT- PCR and immunocytochemical staining. Proliferation test was conducted by using 3 H-thymidine ( 3 H-TdR) incorporation assay, and apoptosis was analyzed by TUNEL, annexin V and hypodiploid apex. Results Compared with wild type K562 and neo-K562, proliferation of p53-K562 decreased by 24.17% and exhibited G 1 arrest. Significant apoptosis did not occur. Conclusion P53 gene could induce less proliferation and G1 arrest but not apoptosis.
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
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