Title: Genetic diversity analysis of primary parent and the first filial generation of pacific white shrimp, Litopenaeus vannamei
Abstract: Two groups of primary parent of Litopenaeus vannamei from Hawaii and their first filial generation have been collected for this experiment. Random amplified polymorphic DNA (RAPD) technique was employed to detect the genetic variation of primary parent and their first filial generation from the population. 16 primers were useful according to the quantity, quality and reproducibility of loci. With the 16 primers, 78 loci of the first group of parent shrimp were, 48 obtained, in which were polymorphic and the proportion of polymorphic loci was 61.54% ; and with the 16 primers there are 89 loci of their first filial generation were obtained, in which 50 were polymorphic and the proportion of polymorphic loci was 56.18% . For the second group, parent had 97 loci with the 16 primers, 49 of which are polymorphic and the proportion of polymorphic loci is 50.52% ; and their first filial generation had 93 loci, 28 of them were polymorphic and the proportion of polymorphic loci was 30.11 % . The average genetic distances of the first group of parent shrimp and their first filial generation were 0.195 9 ?0.039 2, 0.143 5 ?0.026 8, respectively; and for the second group of shrimp those were 0.0922 ?0.018 9, 0.0621 ?0.0148 respectively. There were some different amplified results between the two groups of shrimp with primers OPF - 09, OPZ -11.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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Cited By Count: 1
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