Title: RAPD AND ISSR ANALYSIS ON GENETIC DIVERSITY FOR WILD AND CULTURED OLIVE FLOUNDER PARALICHTHYS OLIVACEUS
Abstract: Randomly Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeat (ISSR) systems were used to detect the genetic diversity in wild and cultured populations of the olive flounder, Paralichthys olivaceus.Thirty individuals from each population were used.Of the 40 RAPD primers screened, 10 generated clear and reproducible bands.A total of 110 reproducible loci ranging from 200 to 2500bp were amplified from all the 60 individuals.The average percentages of polymorphic loci in wild and cultured populations were 59.09% and 60.91%, while the Shannon's diversity indices of the two populations were 16.563 and 16.917, respectively.The intra-population genetic distance was 0.17427 in wild population and 0.17553 in cultured population.Statistic analysis in terms of average percentage of polymorphic loci revealed no significant difference between the two populations (μ= 0.274 μ_ 0.05 =1.96).The genetic distance between the two populations revealed by RAPD analysis was 0.17419.In ISSR analysis, 60 primers were screened and 6 were finally selected for further research.A total of 161 loci were obtained from all the 60 individuals, of which 111 (68.94%) in wild population and 112 (69.57%) in cultured population were polymorphic.The Shannon's diversity indices were 29.254 in wild population and 29.688 in cultured population, respectively.The intra-population genetic distances in wild and cultured populations were 0.19996 and 0.20007, respectively.The genetic distance between the two populations revealed by ISSR analysis was 0.20002.No significant difference in the average percentages of polymorphic loci between the two populations was detected (μ= 0.103 μ_ 0.05 =1.96).The RAPD and ISSR systems produced similar results that the cultured population used in the present study did not show marked decrease in genetic diversity compared with the wild one.Because the cultured population in this study is the first generation offspring of wild caught parents.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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