Title: Effects of Ecdysterone on the Expression of Tumor Necrosis Factor-α and Nuclear Factor κ B in Non-alcoholic Fatty Liver Disease of Rats
Abstract: Objective:To investigate the effect and possible mechanism of ecdysterone on the expression of tumor necrosis factor-alpha (TNF-α) and nuclear factor κ B (NF-κB) in rats with non-alcoholic fatty liver disease of rats.Methods:A total of 36 male Sprague Dawley rats were randomly divided into two groups,who were fed with high-fat diet (experimental group,n=24) and normal basic food (normal control,n=12) respectively.At the end of the 12th week,the experimental group was randomly divided into two subgroups:model group and ecdysterone group,each group contained 12 rats.From the 13th week,the rats in the normal control group and model group were lavaged with normal sodium,and the rats in the ecdysterone group were lavaged with ecdysterone at 10 mg·kg-1·d-1.At the end of the 16th week,all rats were weighed,narcotized,sacrificed,and the liver index,biochemical indicators in serum and liver tissues and the hepatic pathological changes were observed.The expression of TNF-α was detected by ELISA and the expression of NF-κB was measured by immunohistochemical staining.Results:At the end 16th week in ecdysterone group,the serum levels of cholesterol (TC),alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were reduced markedly (2.12±0.58 vs 2.63±0.24 and 53.36±18.48 vs 84.60±36.27,both P0.05;140.20±35.95 vs 243.59±36.38,P0.01);the tissue content of malondialdehyde (MDA) was decreased evidently (184.54±16.45 vs 239.28±23.76,P0.01),while the activity of superoxide dismutase (SOD) was enhanced notably (9.42±0.52 vs 5.18±0.43,P0.01);the liver index was decreased significantly in comparison with that inmodel group (4.35±0.37 vs 5.04±0.46,P0.01);the degree of fatty degeneration and inflammation were relieved dramatically (5.46±0.37 vs 6.30±0.49,P0.01).The expression of TNF-α and the levels of NF-κB were significantly lower (43.04±7.48 vs 61.56±7.27 and 24.65±5.39 vs 45.04±7.46,both P0.01) in ecdysterone group compared with model group.Conclusion:The effects of ecdysterone in preventing NAFLD in rats could be related to the increase of SOD content in hepatic tissue and the decrease of MDA content,tumor necrosis factor-α and NF-κB.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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