Title: Changes and significance of serum nitric oxide levels in the early stage after rat liver transplantation
Abstract: Objective To investigate the changes and significance of serum nitric oxide levels in the early stage after rat liver transplantation.Methods Male Sprague Dawley rats were used as do- nors and recipients of orthotopic liver transplantation,and the time of cold preservation and anhepatic phase was 4 h and 25 min respectively.Forty-eight rats were randomly divided into groups A,B and C (n=16 each).The samples of blood were taken from vena cava and hepatic tissues from left lobe 2 h, 4 h,6 h in groups A,B and C respectively after liver graft reperfusion.ALT and NO levels in serum were detected,and the expression of NF-κB in hepatic tissue was examined by immunohistochemical technique.Amounts of bile flow in 5 min after liver graft reperfused initially were measured.The pathological changes in liver tissues were observed.Results Amounts of bile flow in 5 min in group A (3.73±1.11μl) were greater than those group B (2.35±0.92μl) and group C (2.23±0.81μl) (P0.05).Serum ALT levels in group A (468±36 IU/L) were lower than those in group B (619±49 IU/L) and group C (820±65 IU/L) (P0.05),and serum ALT levels in group B were lower than those in group C (P0.05).Serum NO levels in group A (14.2±1.5μmol/L) were higher than those in group B (10.5±1.2μmol/L) and group C (10.3±1.1μmol/L) (P0.05).Percentage of positive cells expressing NF-κB p65 from hepatic tissues in group A (23.5%±1.9%) was lower than that in group B (43.8%±3.8%) and group C (48.6%±5.1%)(P0.05).Histopathologic examination showed that the injuries in hepatic tissues were aggravated as reperfusion time prolonged, meanwhile,Pearson correlation analysis revealed that the levels of NO were negatively correlated with NF-κB p65 and ALT closely (r:-0.77,-0.74 respectively,P0.01).Conclusion In the early stage after rat liver transplantation,serum NO levels were decreased and the activities of NF-κB enhanced,which resulted in the farther damage of liver function and hepatic tissue structures as reper- fusion time prolonged.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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