Title: Determination of the Content of Quercetin in Xiaoyanliniao Mixture by RP-HPLC
Abstract: OBJECTIVE:To establish RP-HPLC for the determination of the content of Quercetin in the Xiaoyanliniao mixture.METHODS:Samples were Separated on Syltech-500 C18 column(250mm×4.6mm,5μm).The mobile phase consisted of methanol-0.2% phosphoric acid(50∶50)at a flow rate of 1.0mL·min-1.The detection wavelength was 360nm,and the column temperature was 25℃.RESULTS:The linear range of Quercetin was from 19.2 to 96.0μg·mL-1(r=0.999 4).The average recovery was 99.40%(RSD=2.31%,n=6).CONCLUSION:The method was specific,sensitive and reliable in results yet with little interference,and it can be used as quality control for Xiaoyanliniao mixture.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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