Title: Determination of guercetin in shengshitong granule by HPLC
Abstract: Aim To estalish a method for determination of the content of quercetin in shenshitong granule.Methods HPLC method was used.The sample was separated by Kromasil C18 column(250 mm×4.6 mm,5 μm).The mobile phase was methanol-0.4% phosphoric acid(50∶50).The detection wavelength was 370 nm.Flow rate was 1.0 ml·min-1.the column temperature was at 25℃.Results The linear range of quercetin is between 5.20 mg·L-1 and 20.80 mg·L-1.The average recovery is 98.9%(RSD=0.40%,n=9).Conclusions The method is sensitive,rapid and accurate.It is suitable for determining shenshitong grainule.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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