Title: Study on the cloning,sequence analysis and chromosome mapping of bovine ACAS2 gene
Abstract: The liver tissue from Chinese Simmental cattle was collected to extract RNA and the cDNA of the bovine ACAS2 gene was determined and analyzed by homology cloning approach combined with RT-PCR in this study.Sequence analysis and bioinformatics study showed that this partial cDNA contained 1 535 nucleotides.And its basic composition showed 91% and 88% identity with mRNA sequence,ACAS2 gene of the human NM_-13274 and mouse NM_-019811 respectively.The SUNbRH panel was employed to determine the precise location of both three genes.Statistical analysis revealed that,the bovine ACAS2 gene was mapped to the bovine chromosome(BTA) 13 and was closely linked to a framework marker DIK4350 at a 2-point LOD score of 36.4.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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