Title: Molecular Cloning and Sequence Analysis of Porcine Gene KISS-1
Abstract: In order to clone and analyze the gene KISS-1 in various tissues of pig,the total RNA was extracted and mRNA sequence of KISS-1 gene was amplified by RT-PCR method which used two designed primers.The PCR products were ligated into the pMD-19Tvector,and then transformed into competent cells of E.coli DH5α.The sequence was analyzed to identify the recombinant plasmid.The identification analysis showed that the KISS-1 nucleotide sequence shared 81.0%,76.8%,71.4% and 72.4% homology with that of Bos taurus,human,rat and mouse respectively,revealing porcine KISS-1 gene had been successfully cloned in the present study.The sequence has been submitted to GenBank(Accession EU934235).The expression of porcine KISS-1 gene in various tissues was found.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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