Title: MOLECULAR CLONING AND SEQUENCE ANALYSIS OF HYALURONIDASE 2 OF SHEEP
Abstract: According to the published hyaluronidase 2 gene sequences of sheep(NM_001009754),two specific pairs of primers were designed.The Full-length gene of Hyal2 was amplified by overlapping PCR and then was subcloned into PMD19-T vector and sequenced.The Hyal2 gene were1431bp long and contain an open reading frame(ORF) which encoding 476 amino acids.The comparison of the sequences showed that: the homology of Hyal2 gene compared with the template nucleotide was 99.8%,amino acid homology was 99%.Phylogenetic analysis showed that the relationship between target gene and bovine was closer.Bioinformaticsanalysis showed that the protein of the gene was a hydrophilic protein.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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