Title: Establishment of the Clone from Hypocotyl of Lysimachia barystachya
Abstract: The aim was to preserve the wild resources and meet the needs of cultivation,using tissue culture method,hypocotyl of Lysimachia barystachya were used as material to do the research on callus inducement and differentiation,rooting of adventitious buds,rooting multiplication of tube seedlings,transplantation of tube seedlings and stable planting,then the clone from hypocotyl was established.The results indicated that the best medium for callus induction was MS+ZT 0.2mg/L+2,4-D 2.4mg/L;MS+AgNO3 0.5mg/L+KT 1.5mg/L+NAA 0.2mg/L was suitable for callus differentiation;cultivated in the medium White+NO.ABT 2 2.0mg/L after the adventitious buds disposed 24h with IAA 10mg/L was the best method for rooting of adventitious buds;White+NO.ABT 2 2.0mg/L was the best medium for rooting multiplication of tube seedlings;the transplanting survival rate of tube seedlings was 94.2% and stable planting survival rate was 98.3%,the tube seedlings survived and retained all botany characteristics of the wild Lysimachia barystachya.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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