Title: Study on clone establishment of Lobelia sessilifolia
Abstract: With the method of tissue culture,tender stems of Lobelia sessilifolia were used as material to do the research on callus inducement and differentiation,rooting of adventitious buds,ransplantation of tube seedlings and stable planting.MS+KT0.2mg·L-1+2,4-D1.4mg·L-1 was the ideal medium for callus inducing;1/2MS+NAA0.1mg·L-1+ZT0.1mg·L-1+ AgNO30.3mg·L-1was suitable for callus differentiation;the best medium for rooting of adventitious buds and rooting multiplication was 1/2MS+No.ABT2 1.0mg·L-1+NAA0.1mg·L-1+IAA0.2mg·L-1.The transplanting survival rate of tube seedlings was 94.2% and stable planting survival rate was 99%.The tube seedlings stable planted maintained all botany characteristics of the wild Lobelia sessilifolia and beared following year.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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