Title: Vitrificated cryopreservation of bovine oocytes
Abstract: The developmental competences of bovine IVM and GV stage oocytes cryopreserved by vitrification were studied.The results showed that there was no significant differences between TCM199 and PBS in vitrification as the holding medium ( P 0.05),whereas the step of cryopreservation and development stage had significant effects on the development capacity of frozen-thawed bovine oocytes,the development capacity with two step vitrification was better than that with one step vitrification after thawing.The fertilization rates and the cleavage rates of GV oocytes were significantly lower than that of the IVM oocytes( P 0.05).
Publication Year: 2003
Publication Date: 2003-01-01
Language: en
Type: article
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