Title: Preparatory experimental exploration on vitrification of human immature oocytes
Abstract: Objective:To explore the efficiency of vitrification of human immature oocytes.Methods:The human immature oocytes were freezed by OPS vitrification method,then cultured in vitro after thawing,ICSI fecundation,culture conditions were observed and related factors were explored.Results:62 GV and 40 MI oocytes were freezed with OPS vitrification.The survival rates,maturation rates,fertilization rates,cleavage rates of them were 80.65% vs 77.50%,52.00% vs 41.94%,57.69% vs 61.54%,60.00% vs 62.50%,respectively.There was no significant difference in survival rate,maturation rate,fertilization rate,cleavage rate between GV vitrification group and MI vitrification group or between medium HTF group and G-Mops group.There was no significant difference in maturation rate,fertilization rate,cleavage rate of thawed immature oocytes between TCM199 group and IVF-30 group(P0.05).Conclusion:The vitrification of human immature oocytes technology has been initially established.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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