Title: Gene Cloning,Prokaryotic Expression,Purification and Identification of Staphylococcal Enterotoxin A
Abstract: Objective To construct the prokaryotic expression vector of staphylococcal enterotoxin A(SEA) gene and express the gene in E.coli.Methods Amplify SEA gene by PCR,ligate to cloning vector pGEM T-easy,then insert into expression vector pET-30a and transform to E.coli for expression.Results A gene fragment at a length of 770 bp was amplified,and its sequence was identical to that reported.The protein with a relative molecular weight of 31 000 was expressed and identified as SEA after purification.Conclusion SEA protein was successfully expressed.It laid a foundation of further study and application of superantigen SEA.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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