Title: Effect of celecoxib combined with temozolomide on proliferation and apoptosis of glioma cell line U251 cells
Abstract: Objective To study the curative effects of celecoxib combined with antitumor drugs on gliomas and its mechanism. Methods The human glioma cell lines U251 cells were cultured and they treated with celecoxib or temozolomide or both the drugs. The cell morphologic change was observed under the optical microscope. The proliferation of U251 cells was determined by methyl thiaszolyl tetrazoliu assay. The migration ability of U251 cells was determined by wound healing test. The apoptosis of U251 cells were evaluated by flow cytometry with PI-Annexin V staining. The expression of Bcl-2 and Bax in U251 cells were detected by western blot. Results The morphologic changes occurred in U251 cells treated with celecoxib or temozolomide or both the drugs, but the morphologic change in U251 cells treated by both the drugs were most significant. The inhibitory effect of celecoxib combined with temozolomide on U251 cells proliferation was significantly stronger than those of celecoxib or temozolomide (P0.01). The apoptosis rate in U251 cells treated by both the drugs was significantly higher than those in U251 cells treated by celecoxib or temozolomide alone (P0.01). The celecoxib combined with temozolomide more significantly inhibited the migration of U251 cells compared to celecoxib or temozolomide alone. The level of Bax expression was significantly higher and the level of Bcl-2 expression was significantly lower in U251 cells treated by both the drugs than those in U251 cells treated by celecoxib or temozolomide alone. Conclusion The combinative application of celecoxib and temozolomide can promote the apoptosis and inhibit the proliferation and migration of U251 cells in a synergistic manner. These effects of celecoxib combined with temozolomide may be related with the upregnlation of Bax expression and down regulation of Bcl-2 expression.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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