Title: The effect of fluvastatin on the expression of fibronectin in human peritoneal mesothelial cells induced by high-glucose peritoneal dialysate
Abstract: Objective:To explore the effect of fluvastatin(Flu) on the expression of fibronectin(FN) in human peritoneal mesothelial cells(HPMCs) induced by high-glucose peritoneal dialysate(HGPDS).Methods:After incubated in DMEM with 0.01%FBS for 24 h,cultured HPMCs were randomly divided into control group,HGPDS group,HGPDS+Flu group,Flu alone group and DMSO control group.HPMCs were treated for 3,6,12,24,36,48,72 h respectively and the cellular viability were detected by MTT colorimetry.The mRNA expression of FN was observed by RT-PCR.HPMCs were treated for 24 h and the levels of FN in the culture media were measured by ELISA.Results:The results of MTT revealed that lower viability was found in the cells treated with HGPDS and partialy restored in cells treated with different concentrations of Flu at 24 h and 36 h when compared with control group.The significant improvement of cell viability was shown in Flu treatment at concentration of 1 × 10-6 mol/L at the time of 24 h(P 0.05).Compared with the normal control group,the mRNA and protein expressions of FN increased time-dependently in HPMCs treated with HGPDS(P 0.05),which peaked at 6 h and 24 h respectively.The fluvastatin significantly inhibited the effects of HGPDS on the expression of FN in a dose-dependent manner(P 0.05).Conclusion:HGPDS can increase FN expression in HPMCs,which will be inhibited by Flu.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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