Title: Study on the Best Extraction Technology of Leaf Protein from Corchorus olitorius L.
Abstract: Taking the leaves of Corchorus olitorius L.as material,on the basis of single factor tests of solid-liquid ratio,pH values of the extracting solvents,pulping time and soaking time,L9(34) orthogonal experiment was used to optimize the extraction conditions of leaf protein concentrate(LPC) using acid method and heating method.The results showed that the best extraction technology was solid-liquid ratio 1∶8,pH values of the extracting solvents 5,pulping time 3 min,soaking time 6 min.The best optimum precipitating conditions were heating temperature 85℃ for the chloroplastic proteins,the pH 3 for cytoplasmic proteins Ⅰ and the pH 11 for the cytoplasmic proteins Ⅱ.Under these conditions,the extraction rate of LPC reached 8.07% and the yield rate was 37.59%.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot