Title: Study on protein extraction technology from discarded tobacco leaf
Abstract: The optimum extracting conditions of protein in discarded tobacco leaf was studied. The results showed that the optimum technology conditions for tobacco leaf grinding were as follows:the ratio of 1(tobacco leaf):17(water),temperature 60℃,pH 8. 0 and grinding twice with orthogonal experiment design methods Lg(34). The optimum extracting conditions of protein were 60℃ pH 8. 0,60 min and extracting three times with agitation by Response Surface Methodology. And the optimum conditions of depositing protein with acid were pH 3. 0,4℃ for 8 h. The extraction rate reached 76. 62% under the optimal conditions.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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