Title: Determination of monosaccharide composition of polysaccharides in cultured Cordyceps militaris by high performance anion exchange chromatography with pulsed electrochemical detection
Abstract: Objective To determine the monosaccharide compositions of polysaccharides in cultured Cordyceps militaris by high-performance anion-exchange chromatography with pulsed-electrochemical de- tection(HPAEC-PED).Methods Through the application of the hot-water extraction and dilute base ex- traction methods,taking the cultured C.militaris sporophore as raw matarial,six water-soluble polysac- charides were obtained from the cultured C.militaris.The polysaccharides named as P50-1,P70-1,and P70-2 were isolated from the hot-water extraction,and CBP-1,CBP-2,and CBP-3 from the base extrac- tion.The six components of the monosaccharide in the polysaccharides were analyzed by HPAEC-PED. Results The results showed these water-soluble polysaccharides all consisted of mannose,glucose,and galactose except CBP-3,which was supposed to be a glucan.Conclusion This method is simple,high ef- ficiency,accurate and can be used to determine the monosaccharide composition of polysaccharides without derivatization.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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Cited By Count: 2
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