Title: Optimization of liquid culture conditions of a thermo-tolerant xylanase high-producing fungus FL12
Abstract: A thermophilic strain FL12 was isolated from soil samples,and its fermentation conditions of producing thermo-tolerant xyalnase were optimized by single-factor experiment and response surface analysis. The influences of medium composition and fermentation conditions on the fermentation were investigated. The optimal cultivation conditions were including carbon source was corncob at a concentration of 4. 9%,while the optimum nitrogen source was beef peptone(1. 0%). A high xylanase activity of 2284. 06U/mL was obtained after 7d by incubation at 50℃ with initial pH6. 45,medium capacity of 60mL/250mL and rotation rate of 200r/min. The optimum temperature of FL12 crude fermentation broth was 75℃,62. 94% residual enzyme activity was retained at 85℃.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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